Q1.
After three days, observe the surface of the bread or roti carefully using a magnifying glass. Do you notice the growth of mould?
Answer
Yes. By the third day the surface carries cottony white or grey threads, and on them tiny dark pin-heads — black in Rhizopus, greenish-black in Aspergillus. As more days pass the patch spreads and darkens.
Why the moist chamber is built the way it is: mould spores need three things together, and the chamber supplies each one.
- Moisture — from the wet cotton and tissue paper. A dry spore cannot germinate; that is why you add a few drops of water if the bread begins to dry.
- Warmth, 25 – 35 °C — the temperature at which the fungal enzymes work fastest. Below this the spores germinate slowly or not at all, which is exactly why we refrigerate perishable food.
- Nutrients — the starch in the bread or roti, which the hyphae digest and absorb.
Check it yourself: put an identical moist chamber into a refrigerator. After three days it will show little or no mould, while the warm one is covered. Same bread, same water, same spores in the air — only the temperature differs, so temperature must be what made the difference. That single comparison is a fair test.