Q1.
In the school laboratory, observe the available permanent slides of bacteria and cyanobacteria under the microscope.
Answer
Place the slide on the stage, focus first on low power (10×) to find the material, then switch to high power (45×) and finally to the oil-immersion objective (100×) if your microscope has one — bacteria are only a few micrometres across and stay a blur at low power.
What you should see:
- Bacteria — extremely small, stained dots, rods or spirals. Nothing inside them is resolvable: no nucleus, no chloroplast, no visible organelles. Often they lie in chains or clusters.
- Cyanobacteria — noticeably larger, blue-green, usually as unbranched filaments of cells joined end to end (for example Nostoc, Oscillatoria). The colour is spread through the whole cell, not packed into chloroplasts.
Tip: use the fine adjustment only on high power, and reduce the light with the diaphragm — unstained prokaryotes are nearly transparent and are much easier to see in dim, contrasty light than in bright light.